Matthew Reed has released his command line DSK/DMK image utility pair TRSREAD and TRSWRITE. They perform many of the same functions as TRSTools, only on the command line.
TRSREAD is a command line Windows program that can extract files from a TRS-80 virtual disk, with optional flags for including “S”ystem files and “I”nvisible files. You can use wildcards or a list of files you want to extract
TRSWRITE is a command line Windows program that copies files onto TRS-80 virtual disk files.
TRSREAD and TRSWRITE can be downloaded from Matthew Reed’s TRSREAD/TRSWRITE Page.